Probiotics to Eliminate Nitrogenous Metabolites

N. Ranganathan¹, A. Macherone¹, B. Patel1 R. Mehta¹, J. Marczely¹, J. Dickstein¹ and EA. Friedman².
¹Kibow Biotech, Inc. Philadelphia, USA, ²Downstate Medical Center, Brooklyn, USA.
10th International Congress on Nutrition and Metabolism in Renal Disease – poster #P-20

Probiotics proposed to be a component of a unique medical food designed to help maintain normal kidney function are being evaluated. The formulation will assist the kidney in eliminating nitrogenous metabolic end products via the large bowel. This study was designed to evaluate the in situ efficacy of two strains and their ability to metabolize urea, creatinine and uric acid. Lactobacillus sporogenes and L. fermentum were cultured separately on plain agar (PA) containing no added carbon or nitrogen source and PA containing either a) 1.5% urea and 0.5% glucose, b) 1.0% creatinine or c) 1.0% uric acid as the carbon / nitrogen source.

No growth was observed for the strains on PA. L. sporogenes demonstrated growth on PA containing all three of the substrate additives. L fermentum did not grow on any of the substrate media. Samples were taken and compared for residual substrate content. It was determined that L. sporogenes was acclimated to the substrate enriched media and catabolized the substrates via enzymatic activity. In conclusion, acclimated L. sporogenes is a good candidate for use in a medical food to eliminate urea, creatinine and uric acid.